The wheat Phs-A1 pre-harvest sprouting resistance locus delays the rate of seed dormancy loss and maps 0.3 cM distal to the PM19 genes in UK germplasm

نویسندگان

  • Oluwaseyi Shorinola
  • Nicholas Bird
  • James Simmonds
  • Simon Berry
  • Tina Henriksson
  • Peter Jack
  • Peter Werner
  • Tanja Gerjets
  • Duncan Scholefield
  • Barbara Balcárková
  • Miroslav Valárik
  • M J Holdsworth
  • John Flintham
  • Cristobal Uauy
چکیده

The precocious germination of cereal grains before harvest, also known as pre-harvest sprouting, is an important source of yield and quality loss in cereal production. Pre-harvest sprouting is a complex grain defect and is becoming an increasing challenge due to changing climate patterns. Resistance to sprouting is multi-genic, although a significant proportion of the sprouting variation in modern wheat cultivars is controlled by a few major quantitative trait loci, including Phs-A1 in chromosome arm 4AL. Despite its importance, little is known about the physiological basis and the gene(s) underlying this important locus. In this study, we characterized Phs-A1 and show that it confers resistance to sprouting damage by affecting the rate of dormancy loss during dry seed after-ripening. We show Phs-A1 to be effective even when seeds develop at low temperature (13 °C). Comparative analysis of syntenic Phs-A1 intervals in wheat and Brachypodium uncovered ten orthologous genes, including the Plasma Membrane 19 genes (PM19-A1 and PM19-A2) previously proposed as the main candidates for this locus. However, high-resolution fine-mapping in two bi-parental UK mapping populations delimited Phs-A1 to an interval 0.3 cM distal to the PM19 genes. This study suggests the possibility that more than one causal gene underlies this major pre-harvest sprouting locus. The information and resources reported in this study will help test this hypothesis across a wider set of germplasm and will be of importance for breeding more sprouting resilient wheat varieties.

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منابع مشابه

Association mapping and haplotype analysis of the pre - 1 harvest sprouting resistance locus Phs - A 1 reveals a causal

42 Pre-harvest sprouting (PHS) is an important cause of quality loss in many cereal crops 43 and is particularly prevalent and damaging in wheat. Resistance to PHS is therefore a 44 valuable target trait in many breeding programmes. The Phs-A1 locus on wheat 45 chromosome arm 4AL has been consistently shown to account for a significant 46 proportion of natural variation to PHS in diverse mappin...

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Pre-harvest sprouting (PHS) is an important cause of quality loss in many cereal crops and is particularly prevalent and damaging in wheat. Resistance to PHS is therefore a valuable target trait in many breeding programs. The Phs-A1 locus on wheat chromosome arm 4AL has been consistently shown to account for a significant proportion of natural variation to PHS in diverse mapping populations. Ho...

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Association mapping and haplotype analysis of the pre - 1 harvest sprouting resistance locus Phs - A 1 reveals a causal 2 role of TaMKK 3 - A in global germplasm

42 Pre-harvest sprouting (PHS) is an important cause of quality loss in many cereal crops 43 and is particularly prevalent and damaging in wheat. Resistance to PHS is therefore a 44 valuable target trait in many breeding programmes. The Phs-A1 locus on wheat 45 chromosome arm 4AL has been consistently shown to account for a significant 46 proportion of natural variation to PHS in diverse mappin...

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عنوان ژورنال:

دوره 67  شماره 

صفحات  -

تاریخ انتشار 2016